Michal Rurek

Assistant Professor
E-mail: rurek@amu.edu.pl
Phone: +48 61 829 5968
Room 1.105a

Short CV
2000 - PhD in Biology, Adam Mickiewicz University, Poznan
1995 - M.Sc in Biology, Adam Mickiewicz University, Poznan

Research interest

Molecular biology of plant mitochondria - towards understanding of mitochondrial biogenesis under abiotic stress

Main goal

Study of qualitative and quantitative changes in mitochondrial proteome and transcriptome of cauliflower (Brassica oleracea var. botrytis), yellow lupin (Lupinus luteus) and Arabidopsis thaliana under cold and heat stress.

Current scientific interests:

Analysis of the steady-state level of various mitochondrial proteins (subunits of the respiratory chain, CCM proteins involved in the maturation of c-type cytochromes, heat shock proteins and mitochondrial matrix enzymes) in cold and heat stress.
Stress-regulated dehydrin-like proteins in plant mitochondria- cloning, localisation, interactions
Analysis of qualitative and quantitative changes in mitochondrial proteome under stress conditions and after stress acclimation using 2-D electrophoresis (IEF/SDS-PAGE)
Analysis of assembly of mitochondrial complexes and supercomplexes under analysed stress conditions and after stress acclimation (BN-PAGE, BN/SDS-PAGE, DIGE BN/SDS-PAGE)
Study of protein-protein interactions within plant mitochondria in heat stress by means of chemical crosslinking (DSP crosslinker)
Study of plant mitochondrial transcriptome in stress conditions
Bioenergetical properties of cauliflower mitochondria in stress
Mitochondrial morphology in stress

Lectures in: Fundamentals of Molecular Biology; Biochemistry, Molecular Biology & Biotechnology; Mitochondrial Diseases in Humans & Plants
Courses in: Genetic Engineering, Biochemistry, Molecular Biology & Biotechnology
Coordinator in: Methodology of Experimental Biology

Membership and Awards
The regular member of the Polish Society of Experimental Plant Biology
The regular member of the Polish Lupin Society
The regular member of the Polish Biochemical Society
Prof. W. Mozolowski Award - at the 34th Meeting of Polish Biochemical Society in Bialystok (1998)
The best graduated student of A. Mickiewicz University (1995)


Rurek M., Woyda-Ploszczyca A., Jarmuszkiewicz W. (2015) Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, organellar translation and ultrastructure. BBA Bioenerg., in press, doi: 10.1016/j.bbabio.2015.01.005

Rurek M. (2014) Plant mitochondria under a variety of temperature stress conditions. Mitochondrion 19(B):289-294

Rurek M., Galek RA, Jakubowicz M, Pietkiewicz G, Styszko L, Sawicka- Sienkiewicz EJ (2014) Usefulness of RAPD, RFLP and SCAR molecular markers and AGPaseB gene methylation level in the screening of resistance to the golden cyst nematode (Globodera rostochiensis) pathotype Ro1 in different Polish potato genotypes. Australian Journal of Crop Science 8(4):625-630

Augustyniak-Bartosik H, Krajewska M, Weyde W, Madziarska K, Rurek M., Klinger M (2013) Novel PKD1 mutations- the effect on clinical phenotype of ADPKD patients in Lower Silesia. Adv Exp Hyg Med 67:157-163

Galek R., Rurek M., De Jong WS., Pietkiewicz G., Augustyniak H., Sawicka- Sienkiewicz E. (2011) Application of DNA markers linked to the H1 gene conferring potato resistance to pathotype Ro1 of Globodera rostochiensis. J. Appl. Genet. (in press)

Augustyniak-Bartosik H., Krajewska M., Weyde W., Madziarska K., Rurek M., Klinger M. (2011) The effect of novel PKD1 mutations on clinical phenotype of ADPKD patients in Lower Silesian population. Clinical Nephrology (in press)

Rurek M. (2010) Diverse accumulation of several dehydrin-like proteins in cauliflower (Brassica oleracea var. botrytis), Arabidopsis thaliana and yellow lupin (Lupinus luteus) mitochondria under cold and heat stress. BMC Plant Biol 10:181

Augustyniak- Bartosik H., Krajewska M., Weyde W., Mazanowska O., Rurek M., Klinger M. (2008) The phenotypic characteristics of adult polycystic kidney disease have greater impact on the course of progressive disease than the type of mutation of the polycystin 1 gene. Adv Clin & Exp Med, 17: 155-159

Rurek M. (2008) Proteins involved in maturation pathways of plant mitochondrial and plastid c-type cytochromes. Acta Biochim. Polon. 55(3): 417-433

Raczynska KD., Le Ret M., Rurek M., Bonnard G., Augustyniak H. and Gualberto JM. (2006) Plant mitochondrial genes can be expressed from mRNAs lacking stop codons. FEBS Letters 580(24): 5641-5646;

Sawicka-Sienkiewicz E., Galek R., Zalewski D., Augiewicz J., Rurek M., Augustyniak H.(2005) Morphological and molecular characteristics of interspecific hybrids in the genus Lupinus. in: Van Santen E. and Hill GD.: Mexico, Where Old and New World Lupins Meet. International Lupin Association Canterbury, New Zealand;

Pawlowski T., Rurek M., Janicka S., Raczynska KD., Augustyniak H. (2005) Preliminary analysis of cauliflower mitochondrial proteome. Acta Physiol Plantarum 27(3): 317-323;

Stefaniak K., Kokocinski M., Rurek M., Romanowska A., Augustyniak H., Burchardt L. (2005) The polymorphic psa and rbc loci in populations of Plaktothrix agardhii in Polish hypertrophic lakes. Biologia, Bratislava, 60(3): 1-5;

Rurek M., Nuc K., Raczynska KD., Augustyniak H. (2003): Lupin nad9 and nad6 genes: 5' termini of the nad9 gene transcripts differentiate lupin species. Gene 315: 123-132;

Augustyniak H., Lesniewicz K., Rurek M. (2002) Promoters of higher plants mitochondrial genes. Biotechnologia, 3(58): 61-73;

Olczak T., Rurek M., Janska H., Augustyniak H., Sawicka-Sienkiewicz EJ. (2001) Screening of cytoplasmic DNA diversity between and within Lupinus mutabilis Sweet and Lupinus albus sensu lato by restriction fragment length polymorphism (RFLP). J. Appl. Genet. 42(2): 127-137;

Rurek M., Szklarczyk M., Adamczyk N., Michalik B., Augustyniak H. (2001) Differences in editing of mitochondrial nad3 transcripts from CMS and fertile carrots. Acta Biochem. Polon. 48(3): 711-717;

Rurek M. (2000) Mitochondrial genes of complex I NADH dehydrogenase respiratory chain subunits. Editing of the transcripts. Postepy Biochemii 46(2): 163-176 (in Polish);

Pietkiewicz G., Rurek M., Augustyniak H., Stawinski S., Sawicka- Sienkiewicz E. (1998) Application of PCR based molecular markers for the estimation of lupin genetic diversity; DAF, [in:] Lupin in ecological agriculture, Przysiek;

Rurek M., Augustyniak H. (1998) Genes of mitochondrial NADH dehydrogenase of complex I in plants and their expression [in:] Molecular Biology of Mitochondria, AMU Ed. (in Polish);

Rurek M., Oczkowski M., Augustyniak H. (1998) Conservation of the structure and organisation of lupin mitochondrial nad3 and rps12 genes. Acta Biochem. Polon. 45(3): 695-699;

Rurek M. (1994) Editing of organellar tRNA. Postepy Biologii Komorki, 4:391-394 (in Polish)

by michoo